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为探讨小麦化感作用机理,以强化感小麦‘115/青海麦’、‘92L89’和弱化感小麦‘抗10103’材料,设置小麦根水提液浓度为0%、0.2%、1.0%和5.0%的水培试验,测定了看麦娘根系对小麦根水提液化感作用的生理响应。结果表明,小麦根水提液处理显著抑制了看麦娘根的生长,根鲜重抑制率随处理浓度的升高而增大,强化感小麦的抑制率高于弱化感小麦。当处理浓度达5.0%时,不同化感小麦间无显著差异。‘115/青海麦’、‘92L89’和‘抗10103’根水提液处理后,看麦娘的根系活力的抑制率分别为52.0%~59.6%、46.5%~55.0%和27.2%~44.7%,但前二者间无显著差异;看麦娘根系中可溶蛋白含量、SOD、POD、CAT活性及MDA含量显著升高;SOD和POD活性大小均表现为‘115/青海麦’>‘92L89’≈‘抗10103’;CAT活性随处理浓度的升高显著增大,但不同品种小麦间无显著差异。‘115/青海麦’、‘92L89’和‘抗10103’根水提液处理的看麦娘根系MDA含量依次是对照的10.9~25.5倍、5.9~24.2倍和1.2~6.8倍。小麦化感作用引起看麦娘根系细胞膜脂氧化胁迫,并诱导看麦娘根系抗氧化物质类黄酮和总酚含量的合成。可见,降低根系活力、增强保护酶系统活性及抗氧化物质代谢是看麦娘应答小麦化感作用的生理响应。
In order to investigate the allelopathic mechanism of wheat, wheat root water extract concentration was set as 0%, 0.2%, 1.0% and 5.0% to enhance wheat ’115 / Qinghai wheat’, ’92L89’ and weakened wheat ’ % Of hydroponic experiments to determine the physiological response of the allelopathic effect of Amarin root on wheat root water extract. The results showed that the root water extract of wheat significantly inhibited the growth of A. mandshurica roots, the root fresh weight inhibition rate increased with the increase of the treatment concentration, the enhanced sensitivity of wheat was higher than that of the weakened wheat. When the concentration of 5.0%, different allelopathic wheat no significant difference. After treatment with ’115 / Qinghai Mai’, ’92L89’ and ’anti-10103’ water extracts, the inhibitory rates of root activity were 52.0% -59.6%, 46.5% -55.0% and 27.2% -44.7% , But there was no significant difference between the two. The content of soluble protein in the roots of Amaryllis nigra was significantly increased, while the activity of SOD, POD and CAT and the content of MDA were significantly increased. The activities of SOD and POD were all shown as ’115 / Qinghai Mai’> 92L89 ’≈’ anti-10103 ’; CAT activity increased significantly with increasing concentration, but no significant difference between different varieties of wheat. The contents of MDA in the roots of ’Amanita’ treated with ’115 / Qinghai Mai’, ’92L89’ and ’anti-10103’ roots were 10.9 ~ 25.5 times, 5.9 ~ 24.2 times and 1.2 ~ 6.8 times that of the control. Allelopathy of wheat caused the oxidative stress on the membrane of the root of Amaryllis occidentalis, and induced the synthesis of antioxidant flavonoid and total phenolic content in the root of Amaryllis. Visible, reduce the root activity, enhance the activity of the protective enzyme system and antioxidant metabolism is to see Amygdalone physiological response to allelopathy.