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肝细胞核因子(HNF-4)是类固醇/甲状腺素超家族中具有高度保守性的配基依赖型转录因子。HNF-4可在肝、肠、肾和胰腺中表达,并对糖类及脂类等能量物质代谢发挥重要的调节作用。HNF-4可通过其DNA结合区与相应靶基因结合,apoCⅢ就是对HNF-4结合最敏感的靶基因之一。作者通过对apoC Ⅲ的HNF-4结合区的基因分析研究其DNA结合和转录激活性特性。 为了研究apoCⅢ启动子所含的HNF-4反应元件,作者制作了一系列以1. 4 kb启动子片段(p1400CⅢ-luc)开始的突变型和截取物。经HNF-4诱导发现具有前810bp启动子片段(p810CⅢ-luc)的序列可形成最大量激活效应。近来研究已证明在-71～-82间有可与HNF-4结合的DR1样元件
Hepatocyte nuclear factor (HNF-4) is a ligand-dependent transcription factor that is highly conserved in the steroid / thyroid superfamily. HNF-4 is expressed in the liver, intestine, kidney and pancreas and plays an important regulatory role in the metabolism of energy substances such as carbohydrates and lipids. HNF-4 can bind to the corresponding target genes through its DNA binding region. ApoCIII is one of the most sensitive target genes for HNF-4 binding. The authors investigated the DNA binding and transcriptional activation properties of apoC Ⅲ by analyzing the HNF-4 binding region. To investigate the HNF-4 response element contained in the apoCIII promoter, the authors made a series of mutations and intercepts starting with a 1.4 kb promoter fragment (p1400CIII-luc). Upon induction with HNF-4, the sequence with the first 810 bp promoter fragment (p810CIII-luc) was found to produce the greatest amount of activating effect. Recent studies have demonstrated that there is a DR1-like element that binds to HNF-4 between -71 and -82