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目的:建立人胚胎嗅神经鞘细胞(OECs)的体外培养体系。方法:选取3~6个月的流产儿,采用原代培养的方法,在原代培养后的不同时间,用组织免疫化学染色的方法鉴定细胞并计算阳性率。找到细胞数量和阳性率俱佳的时间段,为以后的移植工作打下基础。结果:成功培养出人胚胎OECs,在原代培养后的2～5d主要为巨噬细胞状、多极状,与成纤维细胞很难分别,7～15d主要为扁平的双极、三极形态,突起细长,细胞阳性率可达90%,21d以后为双极、三极形态。结论:自胚胎培养OECs的方法实用可行,方法简单,为利用OECs修复脊髓损伤进入临床提供了条件。
Objective: To establish an in vitro culture system of human embryonic olfactory ensheathing cells (OECs). Methods: Abortion children aged 3 to 6 months were selected. Primary culture was used to identify the cells in different time after primary culture. Immunohistochemical staining was used to identify the positive rate. Find the number of cells and the positive rate of the time period, lay the foundation for future transplant. Results: OECs of human embryos were successfully cultured. The primary cultures were mainly macrophage-like and multipolar from 2 to 5 days after culturing, which was difficult to differentiate from fibroblasts. The flattened bipolar and tertiary forms were observed in 7-15 days, Prominent slender, cell positive rate of up to 90%, after 21d bipolar, three-pole morphology. CONCLUSION: The method of culturing OECs from embryos is practical and feasible, and the method is simple. It provides the conditions for the clinical application of OECs to repair spinal cord injury.