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研究大鼠诱发肝癌与β1,4半乳糖基转移酶(β1,4GT)的相关性。用高效液相层析(HPLC)纯化了荧光标记的受体底物(Gnβ1-2Mα1-6(Gnβ1-2Mα1-3)Mβ1-4Gnβ1-2Gn-PA),用β1,4GT的荧光标记受体底物的HPLC测定方法测定了大鼠诱发肝癌中肝组织的酶活性,发现随着诱癌周数的增加,肝脏酶活性逐渐增高,在第14周癌前期时酶活性达到最高,以后有所回落,但仍保持在高于对照组的水平。同时我们也发现,在胎鼠(孕20d)及出生第一天中酶活性与癌前期相似,说明在肝组织增生期此酶非常活跃。
The correlation between β1-galactosyltransferase (β1,4GT) and liver cancer-induced liver cancer was studied. The fluorescently labeled receptor substrate (Gnβ1-2Mα1-6(Gnβ1-2Mα1-3)Mβ1-4Gnβ1-2Gn-PA) was purified by high performance liquid chromatography (HPLC), and the β1,4GT fluorescence was used to label the receptor substrate. The HPLC method for the determination of the enzyme activity of liver tissue in rats induced by liver cancer found that with the increase of the number of weeks of cancer induction, the activity of liver enzymes gradually increased, and the enzyme activity reached the highest level in the early period of the 14th week, and then fell back. However, it remains at a higher level than the control group. At the same time, we also found that the enzyme activity was similar to that in the precancerous stage in the fetus (pregnancy 20d) and the first day of birth, indicating that this enzyme is very active in the period of liver tissue proliferation.