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[Objective]The mechanism of alkaline phosphatase(ALP) was studied to promote rice-field eel aquaculture industry.[Method] The effects of effectors such as multiple metal ions and organic solvents on ALP in viscera of rice-field eel.[Result]Na+ and K+ didn’t generate big influences on enzyme activity;Mg2+ and Ca2+ could promote ALP while Li+,Cu2+ and Zn2+could restrain ALP enzyme activity.Both HPO42-and WO43-generated by enzyme catalyzing disodium phenyl phosphate possessed strong inhibitory effects on emzyme,and 9.5 mmol/L HPO42-would make enzyme activity decline by 13% while 9.5 mmol/L WO43-would make enzyme decline by 34%.The inhibition types of them were both competitive inhibition on enzyme activity.The organic solvents such as methanol,ethanol,ethylene glycol,isopropanol all generated influences on ALP and the order according to their inhibitory effects was isopropanol>ethanol> methanol>ethylene glycol.[Conclusion]The influences of various effectors on ALP activity of rice-field eel were studied from dynamics perspective to provide theoretical basis for further clarifying ALP mechanism.
[Objective] The mechanism of alkaline phosphatase (ALP) was studied to promote rice-field eel aquaculture industry. [Method] The effects of effectors such as multiple metal ions and organic solvents on ALP in viscera of rice-field eel. [Result] Na + and K + did not generate big influences on enzyme activity; Mg2 + and Ca2 + could promote ALP while Li +, Cu2 + and Zn2 + could restrain ALP enzyme activity.Both HPO42- and WO43- generated by enzyme catalyzing disodium phenyl phosphate possessed strong inhibitory effects on emzyme, and 9.5 mmol / L HPO42-would make enzyme activity decline by 13% while 9.5 mmol / L WO43-would make enzyme decline by 34%. The inhibition types of them were both competitive inhibition on enzyme activity. organic solvents such as methanol, ethanol, ethylene glycol, isopropanol all generated influences on ALP and the order according to their inhibitory effects was isopropanol> ethanol> methanol> ethylene glycol. [Conclusion] The influences of various effectsors on ALP activity of rice-field eel were studied from dynamics perspective to provide theoretical basis for further clarifying ALP mechanism.