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本文通过对中国亚洲玉米螟Ostrinia furnacalis(Guenée)17个地理种群的线粒体COⅡ基因进行序列分析,对中国亚洲玉米螟种群间的遗传分化程度和基因流水平进行了初步研究。在获得的413条序列样本中共发现了34个变异位点、35种单倍型。总体单倍型多样性指数Hd为0.811,种群内单倍型多样度在0.424～0.862范围内。17个种群间的平均基因流(Nm)为2.02。总群体的固定系数Fst为0.234。AMOVA分子方差分析结果表明中国玉米螟的遗传分化主要来自种群内部(76.45%)。各种群的TajimasD值中性检验符合中性突变,说明中国亚洲玉米螟在历史上没有出现群体扩张,群体大小稳定。各地理种群的遗传距离与地理距离间不具有显著的相关性。各地理种群中的单倍型在系统发生树上散布在不同的分布群中,缺乏明显的地理分布格局。
In this paper, the mitochondrial COⅡ gene of 17 geographic populations of Ostrinia furnacalis (Guenée) was sequenced and the genetic differentiation and gene flow of Chinese corn borer (Ostrinia furnacalis) populations were studied. In the obtained 413 sequence samples found a total of 34 mutation sites, 35 haplotypes. The overall haplotype diversity index Hd was 0.811, and the haplotype diversity within the population ranged from 0.424 to 0.862. The average gene flow (Nm) among 17 populations was 2.02. The fixed coefficient Fst of the total population is 0.234. The results of AMOVA molecular variance analysis indicated that the genetic differentiation of Chinese corn borer was mainly from within the population (76.45%). The neutrality test of TajimasD value of all the groups was in line with the neutral mutation, indicating that there was no history of population expansion of China Asian corn borer and the population size was stable. There was no significant correlation between genetic distance and geographic distance of each geographical population. Haplotypes in each geographic population were interspersed in different phylogenetic trees and lacked a clear geographical distribution pattern.