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目的:研究应激刺激对发育期大鼠空间记忆发育的影响。方法:采用发育期Wistar大鼠22只,按随机抽签法分为应激1组(30V)、应激2组(45V)、对照组。电刺激大鼠10次/d,连续1周;采用血清放免法检测生长激素含量变化;通过Morris水迷宫检测应激对大鼠空间记忆能力的影响。结果:应激2组水迷宫空间学习记忆逃逸时间较应激1组犤训练第1～5天应激1,2组分别为(23.61±16.06),(14.19±5.01)s犦,较对照组犤训练第1～5天为(26.86±9.89)s犦,差异有显著性意义(t=2.35～3.02,P<0.05);应激2组的生长激素犤(2.24±0.49)μg/L犦含量偏低,应激1组的生长激素含量犤(3.26±0.82)μg/L犦有明显增高趋势(t=2.83,P<0.05)。结论:应激刺激可提高幼年大鼠空间记忆能力的发育;达到45V应激刺激可以影响幼年大鼠的体格发育,而30V应激刺激则不会妨碍甚至可以促进幼年大鼠的体格发育。
Objective: To study the effect of stress stimulation on spatial memory development in developing rats. Methods: Twenty-two Wistar rats were randomly divided into three groups: stress group 1 (30V), stress group 2 (45V) and control group. The rats were electrostimulated 10 times a day for 1 week. The changes of growth hormone were detected by serum radioimmunoassay. The spatial memory ability of rats was detected by Morris water maze. Results: Compared with the control group, the escape time of spatial learning and memory in water maze of stress 2 group was (23.61 ± 16.06) and (14.19 ± 5.01) s 犦, respectively, (2) There were significant differences between the two groups (t = 2.35 ~ 3.02, P <0.05) in the first to fifth days of training (26.86 ± 9.89) s 犦, but the growth hormone 犤 (2.24 ± 0.49) μg / L 应The content of somatotropin in group 1 was significantly higher than that in group 1 (3.26 ± 0.82) μg / L ((t = 2.83, P <0.05). Conclusion: The stimulation of stress can improve the spatial memory ability of juvenile rats. When stimulated at 45V, it can affect the physical development of juvenile rats. However, the stimulation of 30V does not hinder or even promote the physical development of juvenile rats.