论文部分内容阅读
结核菌感染的流行病学监督越来越迫切,检查外界环境的结核菌是重要手段之一。为发现外界环境的结核菌,一般用生理盐水浸湿的纱条从物体表面收集洗出物作培养,阳性率为9.1～19.1%,并且18～20%有杂菌生长,造成结核菌分离困难。为减少杂菌生长,提高结核菌培养阳性率,作者改进收集培养标本的方法,用5%磷酸钠(Na_3PO_4)液浸湿的纱条,擦拭被检物体表面,再将纱条放在含5ml5%Na_3PO_4的试管内,37℃培养24小时,然后纱条在试管内洗净,获得的洗出物离心沉淀,倒出上
Epidemiological surveillance of tuberculosis infection is more and more urgent. The detection of TB in the external environment is one of the important means. In order to find the TB bacteria in the external environment, it is common to use saline soaked gauze to collect the eluate from the surface of the object for culturing. The positive rate is 9.1% to 19.1%, and 18% to 20% of the bacteria grow. . In order to reduce the growth of bacteria and improve the positive rate of TB culture, the authors improved the method of collecting and culturing specimens, soaked the sliver with 5% sodium phosphate (Na 3 PO 4) solution, wiped the surface of the object under test, % Na_3PO_4 tube, 37 ° C for 24 hours, then the gauze washed in a test tube, the resulting eluate was centrifuged, decanted