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采用多管微电极细胞外记录和微电泳技术，分析了成年大鼠前包氏复合体（ｐｒｅ－Ｂｏｔｃ）区自发放电神经元的放电类型及它们对微电泳甘氨酸（Ｇｌｙ）及其拮抗剂士的宁（Ｓｔｒｙ）的反应。在４３只麻醉、自主呼吸大鼠的ｐｒｅ－Ｂｏｔｃ区共记录到１０４个自发放电神经元。其中呼吸相关神经元（ＲＲＮｓ）５４个，非呼吸相关神经元（ＮＲ－ＲＮｓ）５０个；ＲＲＮｓ包括吸气神经元（ＩＮｓ，ｎ＝２５）、呼气神经元（ＥＮｓ，ｎ＝１７）和跨时相神经元（ＰＳＮｓ，ｎ＝１２）三类。ＩＮｓ包括早吸气型（ｅａｒｌｙ－Ｉ，１１／２５个）神经元，全时程吸气型（Ｉ－ａｌ，１０／２５个）神经元和晚吸气型（ｌａｔｅ－Ｉ，４／２５个）神经元；ＥＮｓ包括全时程呼气型（Ｅ－ａｌ，７／１７个）神经元，早呼气型（ｅａｒｌｙ－Ｅ，６／１７个）神经元，晚呼气型（ｌａｔｅ－Ｅ，４／１７个）神经元；ＰＳＮｓ包括前吸气（ｐｒｅ－Ⅰ，８／１２个）神经元和吸气－呼气跨时相（Ｉ－Ｅ，４／１２个）神经元。微电泳Ｇｌｙ，使受试的２７个神经元中的２２个抑制，少数无反应（ｎ＝３）或兴奋（ｎ＝２），反应呈量效依赖关系。Ｓｔｒｙ使受试的２２个神经元中的８个兴奋，其余１４个无反应，兴奋反应呈量效依?
The multi-tube microelectrode extracellular recording and micro-electrophoresis technique were used to analyze the discharge types of spontaneous discharge neurons in the pre-Botc region of adult rats and their effects on the micro-electrophoresis glycine (Gly) and its antagonists Stry’s reaction. A total of 104 spontaneous discharge neurons were recorded in the pre-Botc area of 43 anesthetized and spontaneously breathing rats. There were 54 respiratory-related neurons (RRNs) and 50 non-respiratory related neurons (NR-RNs). RRNs included inspiratory neurons (INs, n = 25), exhaled neurons Transient phase neurons (PSNs, n = 12) three categories. INs include early-I, 11 of 25 neurons, full-time inspiratory (10/25) neurons and late-I (4/25 (E-al, 7/17) neurons, early-E (6/17) neurons, late- E, 4/17) neurons; PSNs included pre-I (8/12) neurons and inspiratory-expired breath phase (I / E, 4/12) neurons. Gly was electrophoresed in a dose-dependent manner in response to Gly, which inhibited 22 of the 27 neurons tested, a few non-reacted (n = 3) or excited (n = 2) Stry excited the 8 neurons in the 22 neurons under test, and the remaining 14 showed no response, and the excitatory response was dose-dependent.