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应用ＲＴ－ＰＣＲ技术，从人的外周血单个核细胞中扩增出人ＩＬ－６受体的特异性ｃＤＮＡ片段，经琼脂糖电泳证实扩增片段大约为１４００碱基对。运用ＤＮＡ重组技术，将获得的片段连接到ＰＵＣ１８质粒中，经转化、筛选、酶切鉴定构建出重组质粒ｐＵＣＩＬ－６受体；经序列分析证实为编码人ＩＬ－６受体的ｃＤＮＡ片段。
The specific cDNA fragment of human IL-6 receptor was amplified from human peripheral blood mononuclear cells by RT-PCR. The amplified fragment was about 1400 base pairs confirmed by agarose gel electrophoresis. The obtained fragment was ligated into PUC18 plasmid by DNA recombination technique. The recombinant plasmid pUCIL-6 receptor was constructed by transformation, screening and restriction enzyme digestion. The cDNA fragment encoding human IL-6 receptor was confirmed by sequence analysis.