对药典法测定甘草酸含量方法的商榷

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甘草酸是甘草中主要有效成分,由于甘草为临床常用中药,故近年来,文献对甘草酸含量测定方法报导较多,诸如重量法、薄层光密度法、纸层光密度法、气相层析法等。中国药典规定甘草酸含量测定方法为重量法。本文选用薄层光密度法、纸层光密度法和药典法测定了甘草中甘草酸的含量,测定的结果经方差分析和多重分析比较证明,药典法与薄层光密度法和纸层光密度法差异极显著,而后两者之间差异不显著, Glycyrrhizic acid is the main active ingredient in licorice. Because licorice is a commonly used traditional Chinese medicine, in recent years, there have been many reports on the determination of glycyrrhizic acid in the literature, such as gravimetric method, thin-layer densitometry, paper densitometry, and gas chromatography. Law and so on. The Chinese Pharmacopoeia regulates the determination of glycyrrhizic acid as a gravimetric method. In this paper, the content of glycyrrhizic acid in Glycyrrhiza uralensis Fisch was determined by thin layer densitometry, paper densitometry, and pharmacopoeia. The results of the analysis were compared by variance analysis and multiple analysis to prove that pharmacopoeia method and thin layer optical density method and paper layer optical density The difference between the laws is extremely significant, but the difference between the latter two is not significant.
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