Sequences encoding PF4 (58-70) and TSP1(429-459) were linked to yield a single gene TSF which en-codes the fuse-protein of TSF. The gene was cloned into apGEX-2T expression vector to generate a protein GST-TSF,which was strongly expressed in E. coli. The purifiedGST-TSF was degraded with thrombin to generate the pro-tein TSF. With the methods of MTT and wound repair assay,the effects of TSF on the proliferation and migration of ECwere detected, respectively. The results showed that TSFsignificantly suppressed BAEC proliferation and migrationin a dose-dependent manner. The fuse protein GST-TSF, andthe peptides PF4 (58-70) and TSP1 (429-459) also inhib-ited BAEC proliferation and migration, respectively, buttheir inhibition rates were not as high as TSF. Using theCAM assay, it was shown that TSF, GST-TSF, PF4 (58-70)and TSP1 (429-459) inhibited angiogenesis in chick CAMpotentially, the effect of TSF was the highest. In vivo, thegrowth of Lewis lung carcinoma was potently inhibited byTSF treatment, and the inhibition rate was 68.75 % at a doseof 1.00 μmol/kg @ d. These findings suggest that the design onTSF gene was successful, and TSF with its anti-angiogenicand anti-tumor activity, should be a useful source of the in-hibitor.