为建立特异、敏感、快速而简单的结核病免疫诊断技术，制备了８株抗结核分枝杆菌的ＩｇＧ单克隆抗体，经ＥＬＩＳＡ法与结核分枝杆菌及８株非结核分枝杆菌的ＰＰＤ鉴定，筛选了其特异性及敏感性。与结核分枝杆菌聚合ＯＴ测定了其培养上清抗体滴度在１０－２以上。腹水抗体滴度在２×１０－４以上，用免疫转印技术确定了与其特异性结合的抗原相对分子质量分别为４２．７，２７．８ＫＵ。８株杂交瘤细胞的染色体计数为９８～１３０条不等。 To establish a specific, sensitive, rapid and simple immunodiagnosis of tuberculosis, 8 anti-Mycobacterium tuberculosis IgG monoclonal antibodies were prepared by ELISA and Mycobacterium tuberculosis and 8 non-TB Mycobacterium PPD identification, Its specificity and sensitivity were screened. Mycobacterium tuberculosis polymerization OT measured the culture supernatant antibody titers above 10-2. The antibody titer of ascites was above 2 × 10-4. The relative molecular mass of the antigen bound specifically to it was 42.7 and 27.8 KU, respectively, using immunotransfer technique. Eight hybridoma cell chromosome counts ranging from 98 to 130.