目的观察聚维酮碘-乙醇消毒剂对枯草杆菌褐色变种芽孢的杀菌性能。方法实验室采用悬液定量杀菌试验法对其进行研究观察。结果在20℃条件下,不同浓度聚维酮碘-乙醇消毒剂以含有效碘250、500、1 000、2 000、3 000、4 000、10 140mg/L对枯草杆菌黑色变种芽孢作用120 min,杀灭对数值分别为2.867、3.996、5.672、5.516、4.589、2.813、0.680。结论消毒剂杀菌效果随着作用时间延长而增强,但随着浓度的增加先增强后减弱,有效碘浓度1 000mg/L时杀菌效果达到最好。 Objective To observe the bactericidal activity of Povidone-ethanol disinfectant against B. subtilis brown variant spores. Methods Laboratory suspension quantitative disinfection test method to observe the study. Results Under the conditions of 20 ℃, different concentration of povidone-iodine-ethanol disinfectant containing effective iodine 250,500,1 000,2 000,3 000,4 000,10 140mg / L Bacillus subtilis black spores 120 min , Kill the logarithmic value of 2.867,3.996,5.672,5.516,4.589,2.813,0.680. Conclusion The bactericidal effect of disinfectant increased with the prolongation of time, but increased first and then decreased with the increase of concentration. The best bactericidal effect was achieved when the effective iodine concentration was 1 000 mg / L.