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To address the problems existing in testing steam injection profiles in a steam-stimulated well during steam injection and production periods, this paper proposes that the temperature profile in the completion interval could be tested during the soak period. A mathematical model for calculating the vertical distribution of temperature in a single layer reservoir is established based on the temperature characteristics of steam stimulated reservoirs, and the vertical distribution of temperature in a single layer reservoir could be obtained and heat loss could be calculated.The temperature, which is disturbed by thermal conduction in a multilayer reservoir, and heat loss could be derived based on the superposition principle of temperature potential. This paper establishes a multilayer testing temperature profile interpretation method and interprets the actual test temperature profile of Well Gao 3-7-66. The results indicate that the temperature profile in the soak period can reflect the thermal absorption conditions in various reservoir beds.