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[目的 ]扩增和克隆猪带绦虫囊尾蚴 Ag B基因的 c DNA编码区。 [方法 ]提取猪囊尾蚴总 RNA中 ,利用 RT- PCR技术扩增出 Ag B基因 c DNA编码区 ,然后将其克隆到载体 p UC118中进行序列分析。 [结果 ]PCR反应产物为单一条带 ,大小为 2 .6 kb。测序结果与澳大利亚株猪囊尾蚴 Ag B序列有 99.8%的同源性 ,二者的氨基酸序列有 99.3%的同源性。 [结论 ]成功地克隆了猪囊尾蚴 Ag B基因 c DNA编码区。
[Objective] To amplify and clone the c DNA coding region of Ag B gene of Cysticercus cellulosae. [Method] The total RNA of Cysticercus cellulosae was extracted, and the coding region of Ag B gene cDNA was amplified by RT-PCR and cloned into vector pUC118 for sequence analysis. [Result] The PCR product was a single band with a size of 2.6 kb. The results of sequencing showed 99.8% homology with the Ag B sequence of Cysticercus cellulosae in Australia, and the amino acid sequence of the two strains shared 99.3% homology. [Conclusion] The c DNA coding region of Ag B gene of Cysticercus cellulosae was successfully cloned.