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目的通过对比观察青、老年家兔心肌缺血再灌注时外周血中性粒细胞（ＰＭＮｓ）胞浆游离钙（［Ｃａ~（２＋）］ｉ）、丙二醛（ＭＤＡ）及超氧化物歧化酶（ＳＯＤ）的变化规律，探索上述变化对老年兔心肌缺血再灌注损伤的影响特征，并寻找一种可替代心肌组织标本作为判断再灌注损伤的检测指标。方法５年龄及６月龄家兔实验性心肌缺血３０分钟、再灌注３０、９０、３６０分钟，分别取外周血ＰＭＮｓ及心肌组织测定ＭＤＡ、ＳＯＤ、ＰＭＮ［Ｃａ~（２＋）］ｉ和心肌组织钙。结果青、老年组ＰＭＮ［Ｃａ~（２＋）］ｉ、ＭＤＡ于缺血期均明显升高，至再灌注时更显著，而ＳＯＤ活性则明显降低（Ｐ值均＜０．０１）；上述改变在老年组更明显（Ｐ值＜０．０５及０．０１）。ＰＭＮ［Ｃａ~（２＋）］ｉ、ＭＤＡ、ＳＯＤ的变化分别与心肌钙、ＭＤＡ、ＳＯＤ的变化呈正相关（ｒ值分别为０．９２９２、０．９４３６和０．９８６７）。结论钙超载、氧自由基不但共同参与心肌缺血再灌注损伤，且老年期对致损因子更敏感。ＰＭＮｓ指标测定可作为判断心肌缺血再灌注损伤程度的可靠方法。
Objective To compare the changes of cytosolic free calcium ([Ca2 +] i), malondialdehyde (MDA) and superoxide dismutase (SOD) in peripheral blood neutrophils (PMNs) Enzyme (SOD), to explore the characteristics of myocardial ischemia-reperfusion injury in elderly rabbits and to find an alternative to myocardial tissue specimens as indicators to determine the reperfusion injury. Methods Peripheral blood PMNs and myocardial tissue were measured for MDA, SOD, PMN [Ca ~ (2 +)] i and myocardium in experimental and experimental myocardial ischemia of 30 min, 30 min, 30 min, Tissue calcium. Results The levels of PMN [Ca ~ (2 +)] i and MDA were significantly increased in ischemic and reperfused groups, while the activities of SOD were significantly decreased (P <0.01). The above changes In the elderly group was more pronounced (P <0.05 and 0.01). The changes of PMN [Ca ~ (2 +)] i, MDA and SOD were positively correlated with the changes of myocardial calcium, MDA and SOD (r = 0.9292, 0.9436 and 0.9867, respectively). Conclusion Calcium overload, oxygen free radicals not only participate in myocardial ischemia-reperfusion injury, and the elderly are more susceptible to the damage factor. PMNs index determination can be used as a reliable method to determine the extent of myocardial ischemia-reperfusion injury.