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目的制备葛根素固体脂质纳米粒(puerarin solid lipid nanoparticles,Pue-SLN),对Pue-SLN进行体外释药考察,并探讨Pue-SLN对CCl_4诱导的急性肝损伤大鼠的治疗作用。方法采用乳化-超声分散法制备Pue-SLN。大鼠腹腔注射CCl4造成急性肝损伤模型。48只大鼠随机分成6组:正常组、模型组、甘草酸二胺阳性对照组(13.5 mg·kg~(-1))、Pue-SLN高浓度组(27 mg·kg~(-1))、中浓度组(13.5 mg·kg-1)、低浓度组(6.75 mg·kg~(-1))。分别测定大鼠血清中丙氨酸转移酶(ALT)、碱性磷酸酶(ALP)、天门冬氨酸转移酶(AST)的活力,肝组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)含量,计算肝脏指数,并对肝组织进行组织形态学检查。结果 Pue-SLN各剂量组均能抑制肝损伤大鼠血清中ALT、AST、ALP活性,降低肝匀浆中MDA含量,增强SOD、GSH-PX活性,改善肝组织的病理形态。结论 Pue-SLN对CCl_4诱导的大鼠急性肝损伤具有治疗作用。
OBJECTIVE To prepare Puerarin solid lipid nanoparticles (Pue-SLN) for the in vitro release of Pue-SLN and to explore the therapeutic effect of Pue-SLN on CCl_4-induced acute liver injury in rats. Methods Pue-SLN was prepared by emulsion-ultrasonic dispersion. Intraperitoneal injection of CCl4 causes acute liver injury in rats. 48 rats were randomly divided into 6 groups: normal group, model group, glycyrrhizic acid positive control group (13.5 mg · kg -1), Pue-SLN high concentration group (27 mg · kg -1) ), Medium concentration group (13.5 mg · kg -1) and low concentration group (6.75 mg · kg -1). The activities of alanine aminotransferase (ALT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in rat serum were measured respectively. The contents of malondialdehyde (MDA), superoxide (SOD) and glutathione peroxidase (GSH-PX) were measured. The liver index was calculated, and the liver histomorphology was examined. Results Pue-SLN could inhibit the activity of ALT, AST and ALP in serum of rats with liver injury, reduce the content of MDA in liver homogenate, increase the activities of SOD and GSH-PX and improve the pathological changes of liver tissue. Conclusion Pue-SLN has a therapeutic effect on CCl_4-induced acute liver injury in rats.