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1984年Warren Marshall用Warthinstarry(WS)镀银法在胃粘膜内检出幽门弯曲菌(Campylobacter—Pyloriais,CP)之后,该方面研究受到了重视。虽尚未发现其与胃癌的发病有直接关系,但已观察到CP与嗜中性粒细胞浸润程度呈显著正相关。胃粘膜的炎症改变愈明显,CP的感染程度愈重。CP与乙型慢性萎缩性胃炎的发病有密切关系。为了加深该方面的研究,简化染色方法极为必要,本文试用了几种染色方法取得一定效果。取材为咬检人体胃粘膜组织,用10％福尔马林固定,常规石蜡切片4～5μm,组织切片行脱蜡、递度乙醇处理,冲水,用WS方法染色。 WS常规染色方法:在43℃条件下浸于1％硝
Warren Marshall used the Warthinstarry (WS) silver plating method to detect Campylobacter-Pyloriais (CP) in the gastric mucosa in 1984. Although it has not been found to have a direct relationship with the incidence of gastric cancer, a significant positive correlation has been observed between CP and neutrophil infiltration. The more obvious the inflammation changes of the gastric mucosa, the heavier the degree of CP infection. CP is closely related to the onset of chronic atrophic gastritis. In order to deepen the research in this area, it is extremely necessary to simplify the dyeing method. This paper tried several dyeing methods to achieve certain effects. Materials were taken for human gastric mucosal tissue by bite, fixed with 10% formalin, routine paraffin sections 4 ~ 5μm, tissue sections were deparaffinized, treated with ethanol, flushed, stained with WS method. WS conventional dyeing method: immersed in 1% nitrate at 43°C