建立一种测定硫酸软骨素的新方法。十六烷基三甲基溴化铵在KH2 PO4 Na2 HPO4 缓冲溶液中、乳化剂OP共存下与硫酸软骨素反应形成离子对缔合物 ,溶液转变成稳定的乳化液。用光度滴定法测定硫酸软骨素。用标准加入法做回收试验 ,回收率为 99.9%～ 10 0 .2 % ,相对标准偏差0 .32 %～ 0 .4 0 %。方法应用于外贸样品测定 ,结果满意 ,方法准确、简便、快速 To establish a new method for the determination of chondroitin sulfate. Cetyltrimethylammonium bromide in KH2PO4 Na2 HPO4 buffer solution, emulsifier OP coexistence with chondroitin sulfate react to form ion-association, the solution was transformed into a stable emulsion. Determination of Chondroitin Sulfate by Photometric Titration. Recycling test was done by standard addition method. The recoveries were 99.9% ~ 100.2% with relative standard deviations of 0.32% ~ 0.40%. The method is applied to the determination of foreign trade samples, the results are satisfactory, the method is accurate, simple and fast