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介绍利用肝素亲和色谱和高效液相色谱二步法分离纯化基因重组的人血小板第四因子（ｒｈＰＦ４）。采用肝素柱，用含不同浓度的ＮａＣｌ的磷酸盐缓冲液（ｓｏｄｉｕｍｐｈｏｓｐｈａｔｂｕｆｅｒ，即ＰＢＳ）（２０ｍｍｏｌ／Ｌ，ｐＨ７．４）分级洗脱，得到初步纯化的ｒｈＰＦ４（纯度达８０％）；再采用Ｃ１８柱，乙腈（含０．１％三氟醋酸即ＴＦＡ）－水（含０．１％ＴＦＡ）为流动相梯度洗脱，得到进一步纯化的产物。方法快速、准确、分离效果好，经过两次分离后产物纯度达９５％以上。
This article describes the isolation and purification of genetically modified human platelet factor 4 (rhPF4) by two-step heparin affinity chromatography and high performance liquid chromatography. Heparin column was used to fractionate and elute fractions with sodiumphosphatbufer (PBS) (20 mmol / L, pH7.4) containing different concentrations of NaCl to obtain rhPF4 (purity up to 80%). Purified rhPF4 , Acetonitrile (containing 0.1% trifluoroacetic acid or TFA) -water (containing 0.1% TFA) as the mobile phase to obtain the further purified product. The method is rapid and accurate, and the separation effect is good. After two times of separation, the purity of the product reaches more than 95%.