普遍采用的测定受体亲和力和最大结合容量的方法,在放射性配基和受体结合达到平衡后进行,结合复合物和游离配基浓度处于动态平衡,其宏观值不变。但受体测定也可在配基受体结合达到平衡和复合物解离的动态过程中进行,这就是受体的动力学测定法。本文介绍此动力学方程和计算公式(后者以Clark模型假设为基础)并给出一个测定实例。 The commonly used method of determining receptor affinity and maximum binding capacity is performed after the binding of the radioligand and the receptor has been brought to equilibrium, the binding complex and the free ligand concentration are in dynamic equilibrium, and their macroscopic values ​​remain unchanged. However, receptor assays can also be performed during the dynamic process of ligand receptor binding to balance and complex dissociation, which is the kinetic assay of the receptor. This article describes this kinetic equation and calculation formula (the latter based on the Clark model assumption) and gives an example of the determination.