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用培养细胞探讨了各种植物精油对皮下脂肪细胞的影响。方法:将市售白色脂肪细胞置于含有用乙醇稀释的各种精油的培养基中(0.005%),再以含有10μg/mL胰岛素、10μg/mL运铁蛋白、100μM地塞米松、100μM吲哚美辛、1%胎牛血清的DMEM培养基,37℃、5%CO2分化培养7d。计数活细胞数,检
The effects of various plant essential oils on subcutaneous adipocytes were investigated using cultured cells. METHODS: Commercially available white adipocytes were placed in a medium (0.005%) containing various essential oils diluted with ethanol and then contained 10 μg/mL insulin, 10 μg/mL transferrin, 100 μM dexamethasone, and 100 μM helium. Methine and 1% fetal bovine serum in DMEM medium were cultured at 37°C and 5% CO2 for 7 days. Count the number of live cells, check