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Background:Decaffeinated green tea extract(dGTE)can increase fat oxidation during leg exercise,but dGTE is unsuitable for many people(e.g.,those with injuries/disabilities),and its effects on arm exercise and women are unknown.Methods:Eight adults(23-37 years old,4 women)performed an incremental arm cycle test to measure peak oxygen uptake(V02peak),followed by four 1-h trials at 50％V02peak.Subjects were randomly assigned to 650 mg of dGTE or placebo(PLA)for 4 weeks followed by a 4-week washout and crossover trial.Blood samples were obtained pre-exercise and post-exercise for glycerol and free fatty acid analysis.Respiratory gases were collected continuously.Results:V02 showed no differences across trials((0.83-0.89)±(0.19-0.25)L/min,p = 0.460),neither did energy expenditure((264-266)±(59-77)kcal,p = 0.420)nor fat oxidation(dGTE = 0.11 to 0.12 g/min vs.PLA = 0.10 to 0.09 g/min,p = 0.220).Fat oxidation as percentage of energy expenditure was not different for dGTE vs.PLA(23％± 12％to25％± 11％vs.23％± 10％to 21％± 9％,p = 0.532).Glycerol concentra-tion increased post-exercise in all trials,independent of treatments(pre =(3.4-5.1)±(0.6-2.6)mg/dL vs.post =(7.9-9.8)±(2.6-3.7)mg/dL,p = 0.867,if = 0.005 for interaction),as did free fatty acid((3.5-4.8)±(1.4-2.2)mg/dL vs.(7.2-9.1)±(2.6-4.5)mg/dL,p = 0.981,η2 = 0.000).Conclusion:Chronic dGTE supplementation had no effect on lipolysis and fat oxidation during arm cycle exercise in men and women.