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目的 :了解鼻息肉 (NP)中一氧化氮合酶 (NOS)的分布特点和活性及其在NP发病中的作用。方法 :用免疫组织化学法检测 30例NP(NP组 )及 2 0例正常鼻黏膜 (正常鼻黏膜组 )中NOS的表达 ,并用分光光度计法检测其活性。结果 :NP组NOS活性为 (4.0 79± 0 .6 5 5 )U/mg蛋白 ,正常鼻黏膜组为 (1.5 2 6± 0 .310 )U/mg蛋白 ,二者间差异有统计学意义 (P <0 .0 1) ;NP组iNOS有大量细胞表达 ,分布在黏膜上皮、腺体和血管内皮细胞以及炎症细胞中 ,与正常鼻黏膜组比较 ,差异有统计学意义 (P <0 .0 1) ;而eNOS也有表达 ,但与正常鼻黏膜组比较 ,差异无统计学意义 ;NP组i NOS表达明显高于eNOS表达 ,其差异有统计学意义 (P <0 .0 1)。结论 :NP主要表达iNOS ,分布在黏膜上皮、腺体和血管内皮细胞以及炎症细胞中 ,其产生的大量一氧化氮在NP的发病过程中可能起着重要的作用
Objective: To investigate the distribution and activity of nitric oxide synthase (NOS) in nasal polyps (NP) and its role in the pathogenesis of NP. Methods: The expression of NOS in 30 cases of NP (NP group) and 20 cases of normal nasal mucosa (normal nasal mucosa) were detected by immunohistochemistry. The activity of NOS was detected by spectrophotometer. Results: The NOS activity in NP group was (4.079 ± 0.0565) U / mg protein, and that in normal nasal mucosa group was (1.52 6 ± 0.31) U / mg protein, with significant difference between the two groups P <0.01). In iNOS group, a large number of iNOS cells were found in the mucosal epithelium, glandular epithelial cells, vascular endothelial cells and inflammatory cells. Compared with normal nasal mucosa group, the difference was statistically significant (P <0. 0 1), while eNOS also expressed, but there was no significant difference compared with normal nasal mucosa group. The expression of iNOS in NP group was significantly higher than that in eNOS group (P <0.01). CONCLUSION: NP mainly expresses iNOS, which is distributed in mucosal epithelium, glandular and vascular endothelial cells and inflammatory cells. The large amount of nitric oxide produced by NP may play an important role in the pathogenesis of NP