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目的观察百令胶囊对环孢素A(cyclosporin A,CsA)大鼠免疫功能的调节作用。方法 SD大鼠,♂,随机分为对照组、模型组、百令大、小剂量组,每组8只。第12周末用流式细胞仪检测大鼠外周血T细胞亚群(CD4+、CD8+)、B细胞(CD45RA+)及NK细胞(CD161a+)占淋巴细胞的百分比,计算CD4+/CD8+比例。ELISA法测定外周血血清中IgG,IgM含量。结果模型组及百令大、小剂量组大鼠外周血中CD4+与CD8+百分比均稍高于对照组,但差异无统计学意义(P>0.05)。模型组CD4+/CD8+比值显著低于对照组(P<0.05),百令大、小剂量组CD4+/CD8+比值显著高于模型组(P<0.05),百令大剂量组CD4+/CD8+比值显著高于百令小剂量组(P<0.05),其余各组间比较差异无统计学意义。与对照组比较,模型组外周B细胞占淋巴细胞的百分比及血清IgG、IgM含量显著降低(P<0.05或P<0.01);与模型组比较,百令大、小剂量组外周B细胞占淋巴细胞的百分比及血清IgG、IgM含量显著升高(P<0.05,P<0.01);百令大剂量组B细胞(CD3-CD45RA+)比值显著高于百令小剂量组(P<0.05)。结论百令胶囊对CsA所致肾病大鼠免疫失调及低下具有调节和促进作用,大剂量百令胶囊作用更明显。
Objective To observe the effect of Bailing capsule on immune function of cyclosporin A (CsA) rats. Methods SD rats were randomly divided into control group, model group, Bailing large and small dose group, with 8 rats in each group. At the end of the twelfth week, the percentage of T lymphocyte subsets (CD4 +, CD8 +), B cells (CD45RA +) and NK cells (CD161a +) in peripheral blood of the rats were measured by flow cytometry, and the ratio of CD4 + / CD8 + was calculated. Serum IgG and IgM levels in peripheral blood were measured by ELISA. Results The percentage of CD4 + and CD8 + in peripheral blood of model group and Bailing large and small dose groups were slightly higher than that of control group, but the difference was not statistically significant (P> 0.05). The ratio of CD4 + / CD8 + in model group was significantly lower than that in control group (P <0.05). The ratio of CD4 + / CD8 + in Bailing group and small dose group was significantly higher than that in model group (P <0.05) In Bailing low-dose group (P <0.05), the other groups showed no significant difference. Compared with the control group, the percentages of peripheral B lymphocytes and the levels of serum IgG and IgM in the model group were significantly decreased (P <0.05 or P <0.01). Compared with the model group, the percentage of peripheral B cells in the model group was significantly higher than that in the model group (P <0.05, P <0.01). The ratio of B lymphocyte (CD3-CD45RA +) in Bailing high dose group was significantly higher than that in Bailing low dose group (P <0.05). Conclusion Bailing capsule can regulate and promote the immunological imbalance and hypokalemia in CsA-induced nephropathy rats. The effect of high dose Bailing capsule is more obvious.