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目的观察氟伐他汀对体外培养的人Tenon’s囊成纤维细胞增殖的影响,探讨该药作为眼部抗增殖药物的可行性。方法体外培养人Tenon’s囊成纤维细胞,分别给予含1.0×10-9,1.0×10-8,1.0×10-7,1.0×10-6,5.0×10-6,1.0×10-5,2.0×10-5mol.L-1氟伐他汀的10%新生牛血清的DMEM培养基培养24,48,72 h后,应用MTT比色法检测不同作用时间及不同浓度药物对细胞增殖的抑制作用。结果培养细胞的吸光度值随着氟伐他汀浓度的增加及作用时间的延长而降低。作用24 h后,氟伐他汀浓度≥5.0×10-6mol.L-1的各实验组的吸光度值与空白对照组比较均差异有极显著性(均P<0.01);作用48 h后,氟伐他汀浓度≥1.0×10-6mol.L-1的各实验组细胞的吸光度值与对照组比较,均差异有显著性(均P<0.05);作用72 h后,氟伐他汀浓度≥1.0×10-7mol.L-1的各实验组细胞的吸光度值均小于对照组,且均差异有显著性(均P<0.05)。结论氟伐他汀能抑制体外培养的人眼Tenon’s囊成纤维细胞的增殖,且该作用呈剂量和时间依赖性。氟伐他汀有望成为治疗成纤维细胞增殖性眼病的新型药物。
Objective To observe the effect of fluvastatin on the proliferation of human Tenon’s cyst fibroblasts in vitro and to explore the feasibility of this medicine as an ocular antiproliferative drug. Methods Human Tenon’s cystic fibroblasts were cultured in vitro and were given 1.0 × 10-9, 1.0 × 10-8, 1.0 × 10-7, 1.0 × 10-6, 5.0 × 10-6, 1.0 × 10-5, 2.0 × 10-5mol.L-1 fluvastatin 10% fetal bovine serum DMEM medium for 24,48,72 h after MTT colorimetric assay for different time and different concentrations of drug on cell proliferation inhibition. Results The absorbance value of cultured cells decreased with the increase of fluvastatin concentration and the prolongation of action time. After 24 hours, the absorbance values of each experimental group with fluvastatin concentration ≥5.0 × 10-6mol.L-1 were significantly different from the blank control group (all P <0.01) Compared with the control group, the absorbance of cells in each experimental group with statin ≥1.0 × 10-6mol.L-1 showed significant difference (all P <0.05), and the concentration of fluvastatin ≥1.0 × The absorbance values of the 10-7mol.L-1 cells in each experimental group were less than those in the control group, and the differences were significant (all P <0.05). Conclusion Fluvastatin can inhibit the proliferation of human eye Tenon’s cyst fibroblasts cultured in vitro in a dose and time dependent manner. Fluvastatin is expected to become a new drug for the treatment of fibroblast proliferative eye disease.