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真马（Ｅｑｕｕｓ属）首次出现曾被作为第四纪下限标志，近年来不断受到怀疑、甚至否定。然而，根据目前公认的２．５ＭａＢ．Ｐ．的第四纪下限，则真马在欧亚大陆的首次出现正与此界线吻合，从欧洲维拉方动物群、中国北方泥河湾地层、南方元谋盆地，以及其他剖面得到的证据都证明了这一点。真马首次出现事件与２．５ＭａＢ．Ｐ．的许多重大地质事件同时，如古地磁高斯期与松山期界线，中国黄土开始堆积、北极冰盖出现、新构造运动第一次强化等。真马首次出现于２．５ＭａＢ．Ｐ．是由于北极冰盖出现所伴随的海平面下降，导致白令海峡露出水面，欧亚大陆和北美大陆得以连通，在北美起源并在进化上取得成功的真马迅速扩散至欧亚大陆等地。因此，真马首次出现可以作为这些地区第四纪下限的一个显著标志，尤其是在欧亚大陆。
For the first time ever, Equus belonged to the lower limit of Quaternary, which has been constantly under suspicion and even negation in recent years. However, according to the currently accepted 2.5MaB. P. Of the Quaternary lower limit, then the first true horse in Eurasia is coinciding with this line, from the Velafang fauna in Europe, the northern part of China’s Niuhewan strata, the southern Yuanzuo Basin and other evidence obtained by the cross-section have proved at this point. For the first time, Ma incident and 2.5MaB. P. Many major geological events at the same time, such as the ancient geomagnetic period and Matsuyama period line, the Chinese loess began to accumulate, the Arctic ice sheet appeared, the first tectonic movement strengthened. True horse first appeared in 2.5MaB. P. Due to the declining sea level accompanied by the Arctic ice sheet, which led to the appearance of the Bering Strait, the connectivity between Eurasia and North American continent, and the rapid succession to North America origin and evolution. The rapid spread to Eurasia and other places. Therefore, for the first time, the real horse can be a significant indicator of the lower limit of Quaternary in these areas, especially in Eurasia.