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目的 将FHIT基因导入人胰腺癌 1 990细胞并观察其对TNF敏感性的改变 ,探讨FHIT对胰腺癌肿瘤坏死因子 (TNF)敏感性的关系。方法 通过脂质体转染法将外源FHIT导入有FHIT全基因丢失的人胰腺癌 1 990细胞 ,筛选阳性克隆 ,同时以空载体pRC/CMV作为对照 ,加入TNF后作生长曲线并计算细胞成活数。结果 转染FHIT的 1 990细胞阳性克隆与亲代相比 ,其生长速度分别为 32× 1 0 4 cells/well、78× 1 0 4 cells/well(P <0 .0 1 ) ,诱导了 1 990细胞的凋亡 ,且在TNF为 1 0 3μg/L时其细胞存活率分别为 2 3 %、74% (P<0 .0 5)。结论 FHIT基因转染增强了人胰腺癌 1 990细胞对TNF的敏感性 ,FHIT可能介入了TNF的凋亡途径
Objective To introduce human FHIT gene into human pancreatic cancer 1 990 cells and observe the changes of its sensitivity to TNF and to explore the relationship between FHIT and tumor necrosis factor (TNF) sensitivity of pancreatic cancer. METHODS: Human FHIT gene-deleted human pancreatic cancer cell line 1 990 was transfected with exogenous FHIT by lipofection method. The positive clones were screened and the empty vector pRC / CMV was used as a control. After addition of TNF, the cell growth curve was calculated and cell viability was calculated number. RESULTS: The positive clones of 1 990 cells transfected with FHIT had a growth rate of 32 × 104 cells / well and 78 × 104 cells / well (P <0.01) The cell viability was 23% and 74%, respectively (P <0.05) at TNF level of 103μg / L. Conclusion FHIT gene transfection enhances the sensitivity of human pancreatic cancer cell line 1 990 to TNF, and FHIT may be involved in the apoptotic pathway of TNF