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目的:建立HPLC法测定止痛顺气胶囊中盐酸小檗碱含量的方法。方法:色谱柱:Phenomenex Synergi C_(18)(250 mm×4.6 mm,4μm),流动相:乙腈-0.05 mol·L~(-1)磷酸二氢钠溶液(用磷酸调节pH至3.0)(28:72),检测波长:265 nm,流速:1.0ml·min~(-1)。结果:盐酸小檗碱在0.11～1.11μg范围内线性关系良好,r=0.999 9,平均加样回收率为98.2%,RSD=1.8%(n=6)。结论:该方法操作简便,结果准确可靠,可作为止痛顺气胶囊中盐酸小檗碱的含量测定方法。
Objective: To establish a HPLC method for the determination of berberine hydrochloride in Zhitong Shunqi capsule. METHODS: Phenomenex Synergi C_ (18) (250 mm × 4.6 mm, 4 μm) was used as the mobile phase. The mobile phase consisted of acetonitrile-0.05 mol·L -1 sodium dihydrogen phosphate solution : 72), detection wavelength: 265 nm, flow rate: 1.0 ml · min ~ (-1). Results: Berberine hydrochloride showed a good linearity in the range of 0.11-1.11 μg with r = 0.999 9. The average recoveries were 98.2% and RSD = 1.8% (n = 6). Conclusion: The method is simple, accurate and reliable, and can be used as a method for determination of berberine hydrochloride in Zhitong Shunqi Capsules.