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目的探讨人乳头瘤病毒(HPV)16/18感染、p53在女性乳腺浸润性导管癌中的表达及其相互关系。方法采用免疫组化方法和PCR技术,分别检测45例女性乳腺浸润性导管癌、20例乳腺纤维腺瘤及20例正常乳腺组织中HPV16/18 DNA和p53表达水平。结果乳腺癌组HPV16/18 DNA和p53蛋白的表达率(51.1%和46.7%)明显高于乳腺纤维腺瘤组(15.0%和10.0%)和正常乳腺组(5.0%和0%),差异有显著性(P<0.01)。有腋淋巴结转移者HPV16/18DNA和p53蛋白的表达率明显高于无腋淋巴结转移癌者(P<0.05)。随着TMN分期的升高,p53蛋白的表达率明显增加(P<0.05);HPV16/18 DNA与p53蛋白的表达呈明显正相关(rs=0.614,P<0.05)。结论 HPV感染和p53突变共同参与女性乳腺癌的发生和发展,HPV感染可能是促进p53突变的重要因素。
Objective To investigate the expression of human papillomavirus (HPV) 16/18 and their correlation with p53 in female invasive ductal carcinoma of the breast. Methods The expression of HPV16 / 18 DNA and p53 in 45 cases of invasive ductal breast cancer, 20 cases of breast fibroadenoma and 20 cases of normal breast tissue were detected by immunohistochemistry and PCR. Results The positive rates of HPV16 / 18 DNA and p53 protein in breast cancer group (51.1% and 46.7%) were significantly higher than those in fibroadenoma group (15.0% and 10.0%) and normal breast group (5.0% and 0% Significance (P <0.01). The rate of HPV16 / 18 DNA and p53 protein expression in patients with axillary lymph node metastasis was significantly higher than those without axillary lymph node metastasis (P <0.05). With the increase of TMN stage, the expression of p53 protein was significantly increased (P <0.05). HPV16 / 18 DNA was positively correlated with p53 protein expression (rs = 0.614, P <0.05). Conclusion HPV infection and p53 mutation are involved in the occurrence and development of female breast cancer. HPV infection may be an important factor in promoting the mutation of p53.