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目的探讨急性胰腺炎(AP)时花生四烯酸代谢产物血栓素(TXB2)和前列环素(PGI2)的变化及钙拮抗剂异搏定的治疗作用。方法采用十二指肠闭袢法诱发AP大鼠模型,设定AP组和异搏定治疗组,观察2组血浆TXB2、6酮基前列环素1α(6-keto-PGF1α)及其TXB2/6-keto-PGF1α比值的变化,同期行胰腺组织光镜和电镜的检查。结果诱发AP16h和24h,AP组血浆TXB2[(1112±235)pg/ml、(1265±162)pg/ml]和TXB2/6-keto-PGF1α比值[(9·9±0·9)、(10·2±1·3)]均较对照组[(282±56)pg/ml、(315±13)pg/ml,(5·4±2·2)、(5·7±1·8)]明显升高,治疗组血浆TXB2[(671±102)pg/ml、(697±93)pg/ml]及TXB2/6-keto-PGF1α比值[(6·9±2·2)、(6·4±0·7)]较AP组明显降低,同时胰腺组织病理损害程度明显减轻(P<0·05),且花生四烯酸代谢紊乱与胰腺病理损害程度呈正相关(P<0·05)。结论钙拮抗剂异搏定可改善大鼠AP时花生四烯酸代谢紊乱,减轻胰腺的病理损害。
Objective To investigate the changes of arachidonic acid metabolites thromboxane (TXB2) and prostacyclin (PGI2) in acute pancreatitis (AP) and the therapeutic effect of verapamil. Methods AP model was induced by duodenal closure in rats. AP and verapamil groups were set up. The plasma levels of TXB2, 6-keto-PGF1α and TXB2 / 6-keto-PGF1α ratio changes, pancreatic tissue light microscope and electron microscopy examination. Results The levels of TXB2 [(1112 ± 235) pg / ml, (1265 ± 162) pg / ml] and TXB2 / 6-keto-PGF1α in AP group [(9.9 ± 0.9) 10 · 2 ± 1 · 3) were significantly higher than those in the control group [(282 ± 56) pg / ml, (315 ± 13) pg / ml, (5.4 ± 2.2) )] In the treatment group was significantly higher than that in the control group (P <0.01). The ratios of TXB2 (671 ± 102) pg / ml and 697 ± 93 pg / ml] and TXB2 / 6-keto-PGF1α (P <0.05), and the disturbance of arachidonic acid metabolism was positively correlated with the degree of pancreatic pathological damage (P <0 · · · · · · · · ·) 05). Conclusion Verapamil, a calcium antagonist, can ameliorate the metabolic disorder of arachidonic acid in rat AP and alleviate the pathological damage of the pancreas.