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以铁皮石斛品种“云南红杆”为试材,研究了5.1μW/cm2(L1)、10.3μW/cm2(L2)和15.6μW/cm2(L3)3个强度的UV-B辐射处理下,铁皮石斛叶片保护酶活性及MDA含量的变化差异。结果表明:在整个辐射处理期间,L1处理叶片的SOD活性总体高于CK,幅度波动不明显;L2和L3处理的SOD活性总体趋势呈先升高后降低的变化趋势。3个处理组的POD均呈现出先升高后降低的趋势,之后升高再降低。L1处理的CAT活性先缓缓下降,然后逐渐上升,最后趋于平稳;L2和L3的波动幅度较大,最后都明显降低。随着辐射时间的延长,叶片中MDA含量明显增加,如L2和L3。而L1处理下叶片中MDA含量与CK相比,含量变化不大。分析表明,UVB辐射造成抗氧化系统的变化,可能是由于UV-B辐射下活性氧积累导致的。
With Dendrobium candidum “Yunnan red stem ” as test material, UV-B radiation treatment with three intensities of 5.1μW / cm2 (L1), 10.3μW / cm2 (L2) and 15.6μW / cm2 , Dendrobium candidum leaves protective enzyme activity and MDA content changes. The results showed that during the whole radiation treatment, the SOD activity of L1 leaves was generally higher than that of CK, and the amplitude fluctuation was insignificant. The overall trend of SOD activity increased firstly and then decreased after L2 and L3 treatments. POD in the three treatment groups showed a trend of first increasing and then decreasing, then increased and then decreased. The CAT activity of L1 decreased slowly first, then gradually increased and finally stabilized. The amplitude of L2 and L3 fluctuated greatly, and finally decreased significantly. With the extension of radiation time, MDA content in leaves increased significantly, such as L2 and L3. However, the content of MDA in leaves treated with L1 had little change compared with CK. The analysis shows that the changes of antioxidant system caused by UVB radiation may be caused by the accumulation of reactive oxygen species under UV-B radiation.