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目的:研究4-氨基吡啶(4-AP)对人舌鳞状细胞癌(OTSCC)Tca8113细胞体外迁徙和侵袭能力的影响。方法:采用划痕愈合方法及Transwell侵袭实验检测10、20、30 mmol/L 4-AP干预Tca8113细胞后对Tca8113细胞迁徙及侵袭能力的影响。结果:浓度分别为10 mmol/L、20 mmol/L、30 mmol/L 4-AP干预Tca8113细胞后,Tca8113细胞12 h迁徙率分别为(14.07±5.59)%、(11.34±1.79)%及(8.71±3.26)%;24 h的迁徙率分别为(22.20±5.30)%、(14.24±2.52)%及(11.65±2.97)%,均明显小于对照组(19.25±4.50)%和(33.16±6.40)%,差异具有显著性(P<0.01)。细胞侵袭实验结果显示,10 mmol/L、20 mmol/L、30 mmol/L的4-AP作用于Tca8113细胞24 h后穿膜细胞数分别为(112.20±24.36)、(28.87±6.47)、(11.80±5.77)个,随着4-AP浓度的升高,Tca8113穿膜细胞数明显下降,与对照组(179.73±16.14)相比,差异具有显著性(P<0.001)。结论:4-AP具有抑制Tca8113舌鳞癌细胞株体外迁徙侵袭的能力。
AIM: To investigate the effects of 4-aminopyridine (4-AP) on the migration and invasion of human tongue squamous cell carcinoma (OTSCC) Tca8113 cells in vitro. Methods: The effects of 10,20,30 mmol / L 4-AP on the migration and invasion of Tca8113 cells were detected by scratch healing and Transwell invasion assay. RESULTS: The migration rates of Tca8113 cells after treated with 10 mmol / L, 20 mmol / L and 30 mmol / L 4-AP for 12 h were (14.07 ± 5.59)%, (11.34 ± 1.79)% and 8.71 ± 3.26)%. The migration rates at 24 h were (22.20 ± 5.30)%, (14.24 ± 2.52)% and (11.65 ± 2.97)%, respectively, which were significantly lower than that of the control group (19.25 ± 4.50)% and (33.16 ± 6.40) )%, The difference was significant (P <0.01). The results of cell invasion assay showed that the number of transmembrane cells in Tca8113 cells treated with 10 mmol / L, 20 mmol / L and 30 mmol / L 4-AP for 24 h were (112.20 ± 24.36) and (28.87 ± 6.47) 11.80 ± 5.77). The number of Tca8113 transmembrane cells decreased significantly with the increase of 4-AP concentration, which was significantly different from that of the control group (179.73 ± 16.14) (P <0.001). Conclusion: 4-AP can inhibit the migration and invasion of Tca8113 tongue squamous carcinoma cell line in vitro.