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细胞凋亡(apoptosis)又称细胞程序性死亡(programmed cell death)是清除细胞的正常途径,此过程如果发生紊乱将导致细胞发育异常和促进肿瘤的发生。在鼠肝组织和人胸腺组织恶变过程中,已经观察到了细胞凋亡的变化特点,而在口腔粘膜鳞癌中的凋亡特征报道甚少。本文拟采用原位DNA断裂点的3’-羟基末端标记(TUNEL)技术,对口腔正常粘膜、粘膜下纤维性变、白斑和鳞癌的凋亡情况进行检测,以期探讨上皮恶变过程中细胞凋亡的特征性改变。 1 材料与方法 1.1 组织准备 从我院病理科选择28例组织标本,其中正
Apoptosis, also known as programmed cell death, is a normal way to clear cells. If this happens, disorder will lead to abnormal cell development and promote tumorigenesis. In the process of malignant transformation of rat liver tissue and human thymus tissue, the characteristics of apoptosis have been observed, but there are few reports of apoptosis characteristics in oral mucosal squamous cell carcinoma. In this paper, the 3′-hydroxyl end-labeling (TUNEL) technique of in situ DNA breakpoints was used to detect apoptosis in normal oral mucosa, submucosal fibrosis, leukoplakia, and squamous cell carcinoma, in order to investigate the role of cell death in epithelial malignancy. The characteristic change of death. 1 Materials and methods 1.1 Organizational preparation 28 specimens were selected from our department of pathology,