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目的建立人子宫内膜癌SCID鼠模型,为子宫内膜癌的体内实验及临床研究提供合适的动物实验模型。方法分别选择低分化与高分化子宫内膜癌组织标本移植于SCID鼠皮下,原代移植瘤形成后进行鼠间传代,对移植瘤进行相关的生物学检测。结果成功建立3只原代人子宫内膜癌SCID鼠皮下移植瘤模型,其中低分化组织成瘤2只,中分化组织成瘤1只,原代移植成功率为37.5%,自第五代后,移植瘤的传代成功率为100.0%。病理组织学证实:连续传代的移植瘤仍保持原癌组织病理形态特点及人类肿瘤的特点。在子宫内膜癌SCID鼠皮下移植瘤模型中,100.0%移植瘤模型雌激素受体表达为阳性,33.3%孕激素受体表达为阳性,与有关文献报道一致。结论成功建立了人子宫内膜癌SCID鼠皮下移植瘤模型,移植瘤保持了原子宫内膜癌生物学特征,为人类子宫内膜癌的体内实验及临床研究提供了较理想的动物模型。
Objective To establish a human SCID mouse model of endometrial cancer and to provide a suitable experimental animal model for endometrial cancer in vivo and clinical studies. Methods The tissue samples of poorly differentiated and well differentiated endometrial carcinoma were respectively selected and subcutaneously transplanted in SCID mice. After the primary xenografts were established, the passage between the rats was carried out and the related biological tests were performed. Results Three primary human subcutaneous xenograft models of endometrial carcinoma were successfully established. Two of them were poorly differentiated into neoplasms and one of them were differentiated into neoplasm. The success rate of primary transplantation was 37.5%. Since the fifth generation , The success rate of transplanted tumor was 100.0%. Histopathology confirmed: continuous passage of xenografts still maintain the characteristics of the pathological morphology of primary cancer and the characteristics of human tumors. In the subcutaneously transplanted tumor model of SCID mice, the expression of estrogen receptor was positive in 100.0% of the xenografts and 33.3% of the progesterone receptors were positive, which was consistent with the reported in the literature. Conclusion The model of subcutaneously transplanted human endometrial carcinoma in SCID mice has been successfully established. The transplanted tumor maintains the biological characteristics of endometrial carcinoma and provides an ideal animal model for human endometrial carcinoma in vivo and clinical research.