论文部分内容阅读
利用层层自组装技术,成功将氨基苯磺酸、纳米银和辣根过氧化物酶标记的青霉素抗体固定在玻碳电极表面。实现了聚氨基苯磺酸/纳米银放大型电化学免疫传感器的制备,建立了一种高灵敏度检测青霉素的电化学分析方法。辣根过氧化物酶催化过氧化氢氧化还原3,4-二氨基苯甲酸产生电流信号,进行电化学检测,实现蜂蜜中青霉素的定量测定。同时对测定缓冲液的pH、反应温度和时间等进行了优化,最佳条件下,在0.05~6.00μg/L浓度范围内传感器响应电流和青霉素浓度呈良好线性关系,线性方程为Δi(10-5A)=-0.7265C+4.1522,相关系数为0.9946,检出限为1.02μg/L。同时利用该传感器对蜂蜜样品中的青霉素进行了测定,测定结果与酶联免疫法进行了对比,可知该法测定青霉素灵敏度高,具有良好的选择性和稳定性,适用于检测蜂蜜样品中的青霉素残留。
Using layer-by-layer self-assembly technique, aminobenzenesulfonic acid, nano-silver and horseradish peroxidase-labeled penicillin antibody were successfully immobilized on the surface of glassy carbon electrode. The preparation of poly (aminobenzenesulfonic acid) / nano-silver enlargement electrochemical immunosensor was realized, and a high-sensitivity electrochemical method for the determination of penicillin was established. Horseradish peroxidase catalyzed oxidation of hydrogen peroxide reduction of 3,4-diaminobenzoic acid to generate current signals, electrochemical detection, honey quantitative determination of penicillin. At the same time, the pH, reaction temperature and time of the assay buffer were optimized. Under the optimal conditions, the response current of the sensor and the penicillin concentration showed good linearity in the range of 0.05-6.00 μg / L, the linear equation was Δi (10- 5A) = - 0.7265C + 4.1522, the correlation coefficient was 0.9946, the detection limit was 1.02μg / L. At the same time, the sensor was used to determine penicillin in honey samples. The results were compared with enzyme-linked immunosorbent assay. The results showed that penicillin had high sensitivity, good selectivity and stability. The method was suitable for the determination of penicillin in honey samples Residual.