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近年来发展了各种薄层色谱方法来分析自由型胆汁酸和结合型胆汁酸,当一种或几种胆汁酸含量异常时,往往反映了代谢的紊乱或有病变。本文作者在反相高效板(RP-18板)上,用不同流动相研究了十八种普通胆汁酸(8种自由型,4种甘氨酸结合型和6种牛磺酸结合型)的色谱行为,详见下表:标准品及样品均用甲醇溶解,板上迁移距离为6厘米,使用 Desaga 小室,25℃展开。展开后,板在170℃加热10分钟,然后用10%硫酸一甲醇液喷雾,再在170℃加
In recent years, a variety of thin layer chromatography methods have been developed for the analysis of free bile acids and conjugated bile acids. When one or more bile acids are abnormal, they often reflect metabolic disorders or pathological changes. The authors studied the chromatographic behavior of eighteen common bile acids (eight free, four glycine and six taurine bound) on a reversed-phase plate (RP-18 plate) using different mobile phases See the table below for details. Standards and samples are all dissolved in methanol. The migration distance on the plate is 6 cm. Use a Desaga cell and expand at 25 ° C. After deployment, the plate was heated at 170 ° C for 10 minutes and then sprayed with 10% sulfuric acid in methanol, followed by addition at 170 ° C