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目的 观察凡士林纱布厚度、消毒灭菌次数及在锅中各位置的灭菌效果 ,对凡士林纱布的灭菌失败原因进行分析。方法 将嗜热脂肪杆菌芽胞 ATCC795 3布片 ,夹置于各种厚度凡士林纱布的中心放于铝储物盒中 ,经手提式压力蒸汽灭菌器灭菌后做细菌定性分析。结果 经 2次高压灭菌的 32层以内的凡士林纱布中的嗜热脂肪杆菌芽胞 (ATCC795 3)全部杀灭 ,杀灭指数为 5× 10 5～ 5× 10 6。结论 采用手提式压力蒸汽灭菌器灭菌方法可靠 ,灭菌失败原因与凡士林纱布厚度、消毒灭菌次数及在锅中各位置有关 ,应控制凡士林纱布的厚度 ,不易过厚(不超过 32层 ) ,并经 2次高压消毒灭菌 ,置于锅中上层为消毒效果最佳位置。
Objective To observe the thickness of Vaseline gauze, the number of disinfection and sterilization and the sterilization effect in every position in the pot, and analyze the reasons for the failure of Vaseline gauze sterilization. Methods Bacillus stearothermophilus ATCC795 3 cloth, placed in the center of vaseline gauze with various thickness, placed in aluminum storage box, and then the bacteria were qualitatively analyzed by portable pressure sterilizer. Results All strains of Bacillus stearothermophilus (ATCC795 3) in 32 layers of autoclaved Vaseline gauze were completely killed with a killing index of 5 × 10 5 ~5 × 10 6. Conclusions The sterilization method by portable pressure steam sterilizer is reliable. The reason of the failure of sterilization is related to the thickness of Vaseline gauze, the number of sterilization and sterilization and the position in the pan. The thickness of Vaseline gauze should be controlled so as not to be too thick ), And after 2 high-pressure sterilization, placed in the upper pot for the best disinfection.