目前在中国口岸检疫部门使用的大豆疫霉(Phytophthorasojae)常规PCR检测方法存在稳定性不好、检出率不高的局限性。通过在常规PCR的引物两端各增加一条引物设计成套式PCR,并通过对涉及卵孢子DNA提取的各个步骤如取样、细胞破壁、离心等的改进,充分保证了DNA的最高扩增效率和最大提取量。这些步骤的改进使得口岸送检大豆疫霉检出率比常规方法提高了3～4倍。 At present, the routine PCR detection method of Phytophthora sojae used by the quarantine department in China’s port has the limitations of poor stability and low detection rate. The nested PCR was designed by adding one primer to each end of the conventional PCR primer and fully guaranteed the highest amplification efficiency of the DNA by improving various steps involved in the extraction of oospore DNA such as sampling, cell wall breaking, centrifugation and the like, The maximum amount of extraction. The improvement of these steps makes the detection rate of Phytophthora infestans at port increased by 3 ~ 4 times compared with the conventional method.