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以大豆乳清废液为原料,研究了用D101型大孔树脂分离和纯化大豆乳清中大豆异黄酮的工艺条件,分别进行了进行静态和动态吸附与解吸实验。实验结果表明,静态条件为:在pH3、2.0 g大孔树脂/50 mL样品液、吸附时间2.5 h条件下,达到最大吸附率66.4%;在乙醇浓度80%、料液比1:40、洗脱时间5 h,达到最大洗脱率77.7%,在最优工艺条件下回收率达35.6%,得率为0.0072 mg/mL,异黄酮纯度18.94%;动态条件为:最佳上样量为25 mL,流速1.0 mL/min,用90 mL 80%的乙醇基本洗脱完全,可实现较高浓度异黄酮的获得。
Soybean whey liquid waste was used as raw material to study the separation and purification process of soy isoflavones in soy whey with D101 macroporous resin. The static and dynamic adsorption and desorption experiments were carried out. The experimental results showed that the maximum adsorption rate reached 66.4% under the condition of pH3,2.0 g macroporous resin / 50 mL sample solution and adsorption time 2.5 h; at the ethanol concentration of 80% and the solid-liquid ratio of 1:40, 5h, the maximum elution rate reached 77.7%, the recovery rate reached 35.6%, the yield was 0.0072 mg / mL and the purity of isoflavones was 18.94%. The dynamic conditions were as follows: the best loading capacity was 25 mL, the flow rate of 1.0 mL / min, and the complete elution with 90 mL of 80% ethanol can achieve the higher concentration of isoflavones.