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目的:探讨柴胡疏肝散含药血清对衣霉素(TM)诱导的NG108-15细胞轴突生长的影响。方法:实验采用衣霉素对NG108-15神经细胞进行孵育,诱导其形成内质网应激(ERS)细胞模型,分别用正常兔血清、安理申含药兔血清、高、中、低剂量柴胡疏肝散含药血清进行干预,观察各组细胞轴突生长情况。结果:加入柴胡疏肝散含药血清高、中、低剂量孵育干预后,细胞的突起阳性率和平均突起长度明显优于模型组(P<0.01);高剂量组柴胡疏肝散含药血清与低剂量组相比较(P<0.05),与西药安理申组含药血清两者之间无明显差异(P>0.05)。结论:柴胡疏肝散含药血清可促进衣霉素诱导NG108-15细胞内质网应激模型神经元突起的生长。
Objective: To investigate the effect of Chaihu Shugan San-containing serum on tunicamycin (TM) -induced axon growth of NG108-15 cells. Methods: The experiment used tunicamycin to incubate the neuron of NG108-15 and induced the formation of endoplasmic reticulum stress (ERS) cell model. The normal rabbits’ serum, amprenate - containing rabbit serum, high, medium and low dose Hu Shugan powder containing serum intervention, observe the growth of axons in each group. Results: The positive rate and the average protrusion length of the cells were significantly higher than those in the model group (P <0.01) after the intervention of Chaihu Shugan Powder containing high, medium and low doses of the serum. The high dose group of Chaihu Shugan Powder Compared with the low-dose group (P <0.05), there was no significant difference between the drug serum and the drug-containing serum of the western medicine (P> 0.05). Conclusion: Chaihu Shugan San containing serum can promote the growth of neurite outgrowth induced by tunicamycin in the endoplasmic reticulum stress model of NG108-15 cells.