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目的探讨EP4受体拮抗剂ONO-AE3-208对雄激素非依赖性前列腺癌(AIPC)PC3细胞的体外抗肿瘤效应。方法采用RT-PCR检测EP4受体mRNA在PC3细胞中的表达水平,实验组以0.1、1和10μmol/L的ONO-AE3-208作用于PC3细胞,对照组加入同体积的超轻水(ODW),采用MTT比色法、划痕愈合实验和细胞侵袭实验观察ONO-AE3-208对前列腺癌PC3细胞增殖、迁移和侵袭能力的抑制效应。结果 EP4受体在PC3细胞中呈高水平表达;随ONO-AE3-208作用时间和浓度的增加,其对PC3细胞的抑制率没有变化。PC3细胞的实验组细胞迁移比例为(25.30±6.11)%,低于对照组的(40.18±7.25)%(P<0.05)。实验组用0.1、1和10μmol/L的ONO-AE3-208作用于PC3细胞24h后,其穿入下室细胞数分别为(19.72±3.39)、(15.61±3.11)和(10.39±2.15)个/视野,少于对照组的(24.67±3.75)个/视野(P<0.05)。结论 EP4受体在AIPC PC3细胞中表达明显升高。EP4受体拮抗剂ONO-AE3-208对AIPC PC3细胞的增殖没有影响,但对其迁移和侵袭能力有明显的抑制作用。
Objective To investigate the antitumor effect of ONO-AE3-208, an EP4 receptor antagonist, in vitro on androgen-independent prostate cancer (AIPC) PC3 cells. Methods The expression of EP4 receptor mRNA was detected by RT-PCR in PC3 cells. The experimental group was treated with ONO-AE3-208 at concentrations of 0.1, 1 and 10 μmol / L in PC3 cells, and the control group was treated with ODW ), The inhibitory effect of ONO-AE3-208 on the proliferation, migration and invasion of prostate cancer PC3 cells was observed by MTT assay, scratch healing assay and cell invasion assay. Results EP4 receptor was highly expressed in PC3 cells. With the increase of time and concentration of ONO-AE3-208, there was no change in the inhibition rate of PC3 cells. The proportion of PC3 cells migrated in the experimental group was (25.30 ± 6.11)%, which was lower than that in the control group (40.18 ± 7.25)% (P <0.05). The number of penetrating cells in the experimental group after penetrating into PC3 cells with ONO-AE3-208 at concentrations of 0.1, 1 and 10 μmol / L for 24 h were (19.72 ± 3.39), (15.61 ± 3.11) and (10.39 ± 2.15) / Field, less than the control group (24.67 ± 3.75) / field of vision (P <0.05). Conclusion The expression of EP4 receptor in AIPC PC3 cells was significantly increased. ONO-AE3-208, an EP4 receptor antagonist, had no effect on the proliferation of AIPC PC3 cells but significantly inhibited its migration and invasion ability.