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目的:观察长链非编码RNA Linc00472(LncRNA Linc00472)靶向调控微小RNA-381(miR-381)对骨肉瘤细胞增殖、凋亡、迁移、侵袭的影响。方法:收集2017年3月至2018年5月郑州大学第一附属医院收治的骨肉瘤患者29例为研究对象，实时荧光定量聚合酶链反应(RT-qPCR)检测骨肉瘤组织和瘤旁组织中Linc00472的表达。将骨肉瘤U2OS细胞分为pcDNA3.1组、pcDNA3.1-Linc00472组、pcDNA3.1-Linc00472＋miR-NC组、pcDNA3.1-Linc00472＋miR-381组。甲基噻唑基四唑(MTT)检测细胞增殖；流式细胞术检测细胞凋亡；Transwell实验检测细胞迁移及侵袭。双荧光素酶报告实验鉴定Linc00472与miR-381的靶向关系。两组间比较采用独立样本n t检验，多组间比较采用单因素方差分析。n 结果:Linc00472在骨肉瘤组织中的表达水平低于瘤旁组织(0.31±0.03比1.03±0.10，n t＝37.138，n P<0.05)，差异有统计学意义；与pcDNA3.1组比较，pcDNA3.1-Linc00472组细胞存活率[(100.29±10.12)%比(53.29±5.44)%]低于pcDNA3.1组(n t＝12.272，n P<0.05)，差异有统计学意义，迁移细胞数[(266.00±23.61)个比(124.00±12.01)个]与侵袭细胞数[(131.00±13.06)个比(62.00±6.24)个]少于pcDNA3.1组(n t＝16.082，n P<0.05；n t＝14.301，n P<0.05)，差异有统计学意义，而细胞凋亡率[(8.58±0.91)%比(26.61±2.64)%]高于pcDNA3.1组(n t＝19.370，n P<0.05)，差异有统计学意义；miR-381过表达可抑制野生型载体WT-Linc00472细胞的荧光素酶活性(n t＝19.827，n P<0.05)，差异有统计学意义；与pcDNA3.1-Linc00472＋miR-NC组比较，pcDNA3.1-Linc00472＋miR-381组可增强细胞增殖[(53.17±5.33)%比(85.26±8.62)%]、迁移[(122.00±12.31)个比(223.00±22.18)个]及侵袭[(64.00±6.36)个比(104.00±10.20)个]能力高于pcDNA3.1-Linc00472＋miR-NC组(n t＝9.499，n P<0.05；n t＝11.945，n P<0.05；n t＝9.983，n P<0.05)，细胞凋亡率[(26.11±2.62)%比(13.11±1.34)%]低于pcDNA3.1-Linc00472＋miR-NC组(n t＝13.253，n P<0.05)，差异有统计学意义。n 结论:LncRNA Linc00472通过靶向调控miR-381可抑制骨肉瘤细胞增殖、迁移及侵袭并诱导细胞凋亡。“,”Objective:To investigate the effect of long intergenic non-protein coding RNA 472 (Linc00472) targeting microRNA-381 (miR-381) on proliferation, apoptosis, migration and invasion of osteosarcoma cells.Methods:A total of 29 patients with osteosarcoma admitted to our hospital from March 2017 to May 2018 were collected as research objects. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to detect the expression of Linc00472 in osteosarcoma and paraneoplastic tissues. The osteosarcoma U2OS cells were divided into pcDNA3.1 group, pcDNA3.1-Linc00472 group, pcDNA3.1-Linc00472+ miR-NC group, and pcDNA3.1-Linc00472+ miR-381 group. Methyl thiazolyl tetrazolium (MTT) assay was used to detect cell proliferation. Apoptosis was detected by flow cytometry. Transwell chamber experiments were used to detect cell migration and invasion. The dual luciferase reporter assay identified the targeting relationship between Linc00472 and miR-381, and the up-regulated or down-regulated effect of Linc00472 on miR-381 expression by RT-qPCR. The miR-381 mimetic was co-transfected with pcDNA3.1-Linc00472 into U2OS cells to detect cell proliferation, apoptosis, migration and invasion. The expression levels of Cyclin D1, B cell lymphoma/leukemia-2 (bcl-2), p21, bcl-2 associated X protein (bax), matrix metalloproteinase (MMP)-2 and E-cadherin were detected by Western blotting. The comparison between the two groups was done by independent sample n t test, and the comparison between multiple groups by one-way analysis of variance.n Results:The expression level of Linc00472 in osteosarcoma was lower than that in paraneoplastic tissues (1.03±0.10 vs. 0.31±0.03, n t=37.138, n P<0.05). The cell survival rate in the pcDNA3.1-Linc00472 group [(100.29±10.12)% vs. (53.29±5.44)%] was lower than that in the pcDNA3.1 group (n t=12.272, n P<0.05), and the number of migrating cells was [(266.00±23.61) vs. (124.00±12.01) and the number of invasive cells [(131.00±13.06) vs. (62.00±6.24)] were less than that in the pcDNA3.1 group (n t=16.082, n P<0.05;n t=14.301, n P<0.05), while the apoptosis rate was [(8.58±0.91)% vs. (26.61±2.64)%] higher than the pcDNA3.1 group (n t=19.370, n P<0.05). Overexpression of miR-381 could inhibit the luciferase activity of wild-type vector WT-Linc00472 cells (n t=19.827, n P<0.05). The rate of proliferation in pcDNA3.1-Linc00472+ miR-381 group [(53.17±5.33)% vs. (85.26±8.62)%], the number of migrating cells [(122.00±12.31) vs. (223.00±22.18)] and the number of invasive cells [(64.00±6.36) vs. (104.00±10.20)] were increased as compared with the pcDNA3.1-Linc00472+ miR-NC group (n t=9.499, n P<0.05;n t=11.945, n P<0.05;n t=9.983, n P<0.05), and the apoptosis rate was [(26.11±2.62)% vs. (13.11±1.34)%] lower than the pcDNA3.1-Linc00472+ miR-NC group (n t=13.253, n P<0.05).n Conclusion:LncRNA Linc00472 inhibits proliferation, migration and invasion of osteosarcoma cells and induces apoptosis by targeting miR-381.