论文部分内容阅读
中介体复合物在真核生物蛋白编码基因和非编码RNA的转录中非常重要。为了分析大豆中介体亚基的序列特征和基因表达模式,应用BLASTp获取与拟南芥中介体亚基同源的蛋白序列,用Clustal Omega、MAFFT、MEGA等方法进行多序列联配和进化分析,同时应用Me V构建了基因的表达量热图。结果表明:共获得118个基因位点(Locus),186个转录本,在大豆20条染色体上均有分布。Med8的N端具有TBP结构域,Med31的C端具有脯氨酸富集区和假定的核定位信号,Med16的C端有C2-C2型锌指结构域(Zinc-finger motif)。栽培大豆与野生大豆的同源蛋白亲缘关系最近,进化分析符合物种进化规律。大豆不同中介体亚基基因的表达量差异很大,但同一基因在不同组织中的表达差异较小,仅有3个基因在根瘤中特异表达,1个基因在豆荚中特异表达。脱水处理后5个中介体亚基基因表达上调明显,Na Cl处理1和6 h后分别有2和3个中介体亚基基因表达显著上调。这为进一步分析中介体亚基在大豆发育和逆境胁迫中的分子功能奠定基础。
Intermediary complexes are important in the transcription of eukaryotic protein-encoding genes and non-coding RNAs. In order to analyze the sequence characteristics and gene expression patterns of soybean intermediates subunits, BLASTp was used to obtain the homologous protein sequences of Arabidopsis thaliana mediator subunits. Clustal Omega, MAFFT and MEGA were used for multiple sequence alignment and evolution analysis. MeV was used to construct the gene expression calorimetry map. The results showed that a total of 118 Locus and 186 transcripts were obtained, which were distributed on 20 chromosomes of soybean. The N-terminus of Med8 has a TBP domain. The C-terminus of Med31 has a proline-rich region and a putative nuclear localization signal. The C-terminus of Med16 has a Zinc-finger motif. The genetic relationship between cultivated soybean and wild soybean homologous protein is the most recent, and the evolutionary analysis accords with the evolution law of species. The expression of subunit genes in different soybean intermediates varied greatly, but the same gene expression difference in different tissues was small. Only three genes were specifically expressed in nodules and one gene was specifically expressed in pods. After dehydration treatment, the expression of five mediator subunits was significantly up-regulated. After being treated with NaCl for 1 and 6 h, the expressions of two and three mediators, respectively, were significantly up-regulated. This laid the foundation for further analysis of the molecular function of mediator subunits in soybean development and stress.