论文部分内容阅读
鉴定主栽水稻品种及育种骨干亲本的抗瘟基因型,有助于了解不同抗病基因在品种中的分布,为抗病品种选育及品种布局提供参考。本研究选取辽宁省24份水稻材料,根据7个抗稻瘟病基因的保守区设计引物,扩增各品种的编码区序列,对扩增的序列进行对比分析,鉴定各基因在24个品种中的分布情况。结果表明:辽宁省24个主栽水稻品种均不携带Pi21、Pi36、Pi37或其抗病等位基因,而Pid2、Pid3、Pita和Pik/Piks/Pikm/Pikp在24个品种中以不同突变类型及不同频率出现,其中,在2个品种中检测到Pid2抗病基因及Pid3的抗病等位基因;4个品种检测到Pita的抗病等位基因,Pita的等位基因中新发现的几处碱基突变并未影响抗病基因的功能;所有品种中均无与Pik及其复等位基因完全一致的序列,但利用携带AVR-pik的稻瘟病菌接种鉴定结果表明,辽粳454和沈农265携带的Pik等位基因可能仍具有抗病基因功能。
Identification of main rice cultivars and breeding parents of the anti-blast genotypes help to understand the distribution of different resistance genes in breeds and provide a reference for the breeding of resistant varieties and variety distribution. In this study, 24 rice cultivars from Liaoning Province were selected. Primers were designed based on conserved regions of seven blast resistance genes. The coding region sequences of each variety were amplified. The amplified sequences were compared and identified. Among the 24 cultivars Distribution. The results showed that Pi21, Pi36, Pi37 or their disease-resistant alleles were not carried in all the 24 main cultivated rice varieties in Liaoning Province. Among the 24 varieties, Pid2, Pid3, Pita and Pik / Piks / Pikm / And Pseudoscinnebraceae (Pid2) were detected in two cultivars. The resistance alleles of Pid2 and Pid3 were detected in two cultivars. Among the four cultivars, Pita alleles and Pita alleles were newly detected The base mutation did not affect the function of disease-resistant genes. There was no sequence identical with Pik and its allele in all varieties. However, the results of inoculation identification with Magnaporthe grisea carrying AVR-pik showed that Liaojing 454 and The Pik allele carried by Shennong 265 may still have resistance gene function.