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目的探讨4℃保存对人悬浮红细胞免疫功能的影响。方法采集健康献血者静脉血,制备悬浮红细胞,分别于保存前及4℃条件下保存1、2、3、4、5、7和9周时取样2 ml,采用直标法流式细胞术检测红细胞补体受体1(CR1)、白细胞分化抗原59(CD59)分子水平。结果红细胞CR1-GMFIR随4℃保存时间延长有逐渐增加的趋势,至4周时达最高值,之后逐渐下降,组间差异无统计学意义(P>0.05),与4℃条件保存前相比,除4周时CR1-GMFIR明显升高外,其他时间点的差异无统计学意义;而红细胞CD59-GMFIR呈逐渐下降趋势,组间差异有统计学意义(P<0.01),3~9周时CD59-GMFIR均明显低于4℃条件保存前。结论 4℃条件保存悬浮红细胞损害红细胞免疫功能,对红细胞CR1-GMFIR影响小,对CD59-GM-FIR影响大;保存5周内二指标受影响较小,保存2~3周时受影响最小。
Objective To investigate the effect of 4 ℃ preservation on human erythrocyte immune function. Methods Venous blood from healthy blood donors was collected to prepare suspension erythrocytes. Two ml samples were collected before storage at 1, 2, 3, 4, 5, 7, and 9 weeks before storage and were detected by direct flow cytometry Erythrocyte complement receptor 1 (CR1), leukocyte differentiation antigen 59 (CD59) molecular level. RESULTS: The CR1-GMFIR of erythrocytes tended to increase with the prolongation of storage time at 4 ° C, reaching the highest value at 4 weeks and then gradually decreasing. There was no significant difference between the two groups (P> 0.05) The levels of CD59-GMFIR in erythrocytes decreased gradually at 4 weeks (P <0.01), while CD59-GMFIR in erythrocytes showed a statistically significant difference (P <0.01) -GMFIR were significantly lower than the 4 ℃ conditions before preservation. CONCLUSION: The preservation of erythrocyte immune function by the suspension of erythrocytes at 4 ℃ has little effect on the CR1-GMFIR of erythrocytes and has a great effect on CD59-GM-FIR. The two indexes are less affected after 5 weeks of storage and are the least affected when stored for 2-3 weeks.