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目的探讨江西儿童哮喘与GSDML、ORMDL3基因多态性的关系。方法采用序列特异性引物聚合酶链反应(PCR-SSP)及测序技术检测儿童哮喘组和正常组ORMDL3(rs4378650)、GSDML(rs7216389)基因型。计算各组基因型频率及等位基因频率,并进行对比分析。结果携带C等位基因(rs4378650)的个体发生哮喘的几率明显高于对照组(P<0.05),携带T等位基因(rs7216389)的个体发生哮喘的频率与对照组差异无统计学意义(P>0.05)。结论 ORMDL3基因多态性可能会导致儿童哮喘,具有一定的相关性。
Objective To investigate the relationship between childhood asthma and GSDML and ORMDL3 gene polymorphisms in Jiangxi Province. Methods ORMDL3 (rs4378650) and GSDML (rs7216389) genotypes in children with asthma and normal controls were detected by PCR-SSP and sequencing. The genotype frequency and allele frequency of each group were calculated and compared. Results The incidence of asthma in individuals carrying the C allele (rs4378650) was significantly higher than that in controls (P <0.05). There was no significant difference in the frequency of asthma between individuals with the T allele (rs7216389) and controls (P > 0.05). Conclusion ORMDL3 gene polymorphism may lead to asthma in children, with a certain correlation.