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目的:观察消乳块胶囊对二甲基苯蒽(DMBA)诱发SD大鼠乳腺癌的影响及其机制。方法:50天龄Sprague-Daw ley系健康雌性未孕大鼠30只随机分为3组:模型组、治疗组和对照组。模型组和治疗组大鼠均先经两次DMBA(15mg溶于1.5mL芝麻油,间隔1周)灌胃造模,同时治疗组大鼠以消乳块胶囊0.4g/(kg.d)灌胃,持续给药,在第14周处死。大鼠乳腺组织及肿瘤组织均作常规病理,免疫组化检测ERα、ERβ、PR、AhR的表达,酶联免疫吸附法测定血浆E2、P、LH、FSH水平。结果:治疗组癌变率明显低于模型组(P<0.05),分别是25.0%(2/8)和71.4%(5/7)。同对照组相比,ERβ、PR和AhR在治疗组和模型组中升高明显(P<0.05~P<0.01),ERβ在治疗组升高更为明显,同模型组相比,差异仍有显著性(P<0.05)。血清激素测定显示,同对照组和模型组相比,E2、FSH和LH在模型组中升高明显(P<0.05~P<0.01);治疗组E2、FSH仍高于对照组(P<0.05~P<0.01)。结论:消乳块胶囊对DMBA诱导的大鼠乳腺恶性肿瘤有预防作用,其机制可能是通过ERβ介导的保护作用和调节体内激素水平来实现。
OBJECTIVE: To observe the effect and mechanism of Xiaomilai capsule on breast cancer in SD rats induced by dimethyl benzoquinone (DMBA). Methods: A total of 30 Sprague-Dawley Sprague-Dawley healthy female non-pregnant rats were randomly divided into 3 groups: model group, treatment group and control group. Rats in both the model group and the treatment group were given intragastric administration of DMBA (15 mg in 1.5 mL sesame oil at intervals of 1 week), and the rats in the treatment group were given intragastric administration with Xiaodan block capsule 0.4 g/(kg.d). The drug was administered continuously and was sacrificed in the 14th week. Rat mammary tissue and tumor tissue were used for routine pathology. The expression of ERα, ERβ, PR, and AhR was detected by immunohistochemistry. Plasma E2, P, LH, and FSH levels were determined by enzyme-linked immunosorbent assay. Results: The cancer rate in the treatment group was significantly lower than that in the model group (P<0.05), which was 25.0% (2/8) and 71.4% (5/7), respectively. Compared with the control group, ERβ, PR, and AhR increased significantly in the treatment group and the model group (P<0.05-P<0.01). ERβ increased more significantly in the treatment group, and there was still a difference compared with the model group. Significant (P < 0.05). Serum hormone assays showed that E2, FSH, and LH were significantly higher in the model group than in the control and model groups (P<0.05-P<0.01); E2 and FSH were still higher in the treatment group than in the control group (P<0.05). ~P<0.01). Conclusion: Xiaomiling Capsule can prevent DMBA-induced mammary tumors in rats. The mechanism may be through the protective effect of ERβ and regulation of hormone levels in vivo.