建立了液相色谱-串联质谱法测定人血浆中的匹维溴铵。以紫杉醇为内标,采用C18色谱柱,流动相为乙腈-2 mmol/L乙酸铵溶液-甲酸(70:30:0.1),多反应监测(MRM)扫描,监测离子对m/z 512.5→231.3(匹维溴铵)和m/z 854.3→286.3(紫杉醇)。低、中、高浓度匹维溴铵的血浆提取回收率分别为111.7%、104.9%和99.7%,日内和日间RSD均小于15%。 A liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) method was developed for the determination of pinacolium bromide in human plasma. Paclitaxel was used as an internal standard. The mobile phase consisted of acetonitrile-2 mmol / L ammonium acetate solution-formic acid (70:30: 0.1) and multiple reaction monitoring (MRM) with C18 column. The ion pair m / z 512.5 → 231.3 (Pirivironium bromide) and m / z 854.3 → 286.3 (paclitaxel). The plasma extraction recoveries of low, medium and high concentrations of pivalivirubine were 111.7%, 104.9% and 99.7%, respectively. The intra-day and inter-day RSDs were less than 15%.